Novel_miR218对山羊外周血单核细胞中SLAM受体表达的影响

doi:10.11843/j.issn.0366-6964.2018.11.015

Abstract

Abstract:

In order to study the effect of novel_miR218 on the expression of signaling lymphocyte activation molecule (SLAM), the receptor of Peste des petits ruminants virus (PPRV) on goat lymphocytes, we detected the expression levels of SLAM, miR218 and viral loads in goat peripheral blood mononuclear cells (PBMCs) infected with PPRV vaccine virus N75-1 strain by using Western blot assay and quantitative PCR analysis. Then, we detected the levels of SLAM expression and viral loads in PPRV-infected goat PBMCs pre-transfected with miR218 mimic or miR218 inhibitor. The results showed that the cytopathic effect (CPE) can be detected in infected cells at 24 h post infection (hpi) with progressive increase in CPE at 48-72 hpi. In compared with the mock infected group, the expression fold of SLAM mRNA in PPRV infected group increased significantly at 24 hpi (P<0.01) followed by a gradually decreased levels, while the expression level of novel_miR218 decreased significantly at 24 hpi (P<0.01) and then gradually increased. In the uninfected control group, the expression level of novel_miR218 and SLAM mRNA showed no significant change at each detected time point. The expression pattern of SLAM protein in the infected group was similar to that of the mRNA, while an increased levels of PPRV-N protein was observed. The expression levels of SLAM and PPRV-N in miR218 mimic transfected group were significantly lower (P<0.05 or P<0.01) than that in the uninfected control group in a dose-dependent manner, while the expression levels of SLAM and PPRV-N in miR218 inhibitor transfected group were significantly higher (P<0.05 or P<0.01) than those in control group in a dose-dependent manner. This study indicated that the expression of SLAM was closely correlated with the miR218 expression in PPRV infected goat PBMCs, and the results of transfection of cells with miR218 mimic or inhibitor suggested that miR218 can negatively regulate the expression of SLAM and virus replication in PPRV infected PBMCs.

Key words: novel_miR218, PPRV, goat PBMCs, SLAM

CLC Number:

S852.659.5

SONG Hua-jie, WANG Ting, LI Zhen, WANG Jing-yu, QI Xue-feng. The Effects of Novel_miR218 on the Expression of SLAM in Goat Peripheral Blood Mononuclear Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(11): 2435-2441.

References

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The Effects of Novel_miR218 on the Expression of SLAM in Goat Peripheral Blood Mononuclear Cells

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