羊布鲁菌体内诱导基因的筛选与初步鉴定

doi:10.11843/j.issn.0366-6964.2018.09.019

Abstract

Abstract:

In this study, we aimed to screen and identify in vivo-induced antigens of Brucella melitensis via in vivo-induced antigen technology (IVIAT), which may provide a scientific basis for selecting of novel virulence molecules, diagnostic targets, vaccine candidate antigens and drug targets for Brucellosis control. The probe was prepared by absorbing the clinical positive serum collected from Brucella-infected animals onto PVDF membrane. Expression library of 16M gene of B. melitensis was constructed for identifying in vivo-induced antigens. The identified genes were confirmed by RT-PCR and the PCR products were sequenced and analyzed using bioinformatic software. The results showed that 14 of in vivo-induced genes were obtained from expression library of 16M gene of B. melitensis by IVIAT using the probe prepared in this study. Further analysis using bioinformatic software demonstrated that the products of these genes are mainly involved in the biological processes of glucose metabolism, tRNA modification, ion transport and transmembrane transport of B. melitensis. Some of these molecules have been identified as drug targets and virulence molecules in Brucella spp, while some may be related to Brucella virulence and immunity. The IVIAT was successfully used to the screening and identification of in vivo-induced antigens of B. melitesis. The in vivo-induced antigens of B. melitesis identified in this study are new candidate antigens and diagnostic markers for vaccine and diagnostic products of B. melitesis.

Key words: Brucella, in vivo-induced antigen technology(IVIAT), RT-PCR

CLC Number:

S852.614

YANG Yan-ling, CHENG Yue-ning, ZHANG Ping, ZHOU Yu-cheng, ZHANG Hai-wei, CHENG Shi-peng. Screening and Preliminary Identification of in vivo-induced Genes of Brucella melitensis[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1979-1986.

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Screening and Preliminary Identification of in vivo-induced Genes of Brucella melitensis

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