广东省Ⅰ型鸭疫里氏杆菌分离鉴定及PFGE分型研究

doi:10.11843/j.issn.0366-6964.2018.09.018

Abstract

Abstract:

The objective of this study was to investigate the prevalence of serotype Ⅰ Riemerella anatipestifer isolates in Guangdong province and obtain the molecular typing characteristics. R.anatipestifer strains were isolated from brain or liver samples collected from sick and dead ducks or geese during 2007 to 2017, and then these strains were subjected to purification and identification by PCR method. R. anatipestifer serotype Ⅰ were identified by glass aggregation method and tested for pulsed field gel electrophoresis (PFGE) to analyze the homology by clustering analysis with BioNumerics 6.0 software. Results were as follows:A total of 220 R. anatipestifer isolates were collected in our laboratory during 2007 to 2017, and 119 R. anatipestifer isolates were serotype Ⅰ, accounting for 54.09% of all isolated strains. PFGE genotyping showed that R. anatipestifer serotype Ⅰ isolates were differentiated into thirty-three different PFGE types (with similarity percentage of ≥ 85%), including nineteen clusters and fourteen single types. In conclusion, the predominate serotype of R. anatipestifer isolates in Guangdong province was serotype Ⅰ. Serotype Ⅰ R. anatipestifer isolates showed diverse genotyping band patterns. Low degree of homology was observed among these isolates and there was a clonal spread on a small scale.

Key words: Riemerella anatipestifer, serotype, pulsed field gel electrophoresis

CLC Number:

S852.61

GUO Jing-ying, LI Ya-fei, JIANG Hong-xia, ZENG Zhen-ling. Analysis and PFGE Genotyping of Serotype Ⅰ Riemerella anatipestifer in Guangdong Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(9): 1969-1978.

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Analysis and PFGE Genotyping of Serotype Ⅰ Riemerella anatipestifer in Guangdong Province

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