酿酒酵母细胞壁诱导体外培养绵羊瘤胃上皮细胞表达SBD-1的研究

doi:10.11843/j.issn.0366-6964.2018.05.007

Abstract

Abstract:

In present study, we aimed to explore the effect of the cell wall of Saccharomyces cerevisiae (S. cerevisiae) on the expression of sheep beta defensin-1 (SBD-1) in primary culture of sheep's ruminal epithelial cells (SRECs). Firstly, the cell wall of S.cerevisiae was extracted and subjected to quality test. Then, SRECs were cultured and stimulated with cell wall of S. cerevisiae (0, 25, 50, 100, 200, 400 μg·mL^-1) for different times (0, 2, 4, 8, 12, 24 h). RT-PCR and ELISA were performed to grope the optimal concentration and time for SBD-1 expression. The results showed that:1) The cell wall of S. cerevisiae extracted was pure and suitable for the subsequent experiments; 2) SRECs were in vitro cultured successfully (cobblestone pavement), that meet the experimental requirement; 3) Compared with other concentration groups, the expression of SBD-1 mRNA and protein reached the highest when the cell wall of S. cerevisiae of 200 μg·mL^-1 was used to stimulate SRECs for 12 h (P<0.001). The results indicated that the cell wall of S. cerevisiae could improve the expression of SBD-1 in SRECs, and optimal condition was 200 μg·mL^-1for 12 h.

Key words: S. cerevisiae, cell wall, sheep, ruminal epithelial cells, β-defensin-1

CLC Number:

S826.2

TIAN Qiao-zhen, JIN Xin, ZHANG Man, ZHANG Zhao-yi, WANG Yun-he, YANG Yin-feng. Effects of Cell Wall of Saccharomyces cerevisiae on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(5): 927-934.

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Effects of Cell Wall of Saccharomyces cerevisiae on the Expression of SBD-1 in Cultured Ruminal Epithelial Cells of Sheep

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