影响猪树突状细胞对猪细小病毒样颗粒提呈方式的因素

doi:10.11843/j.issn.0366-6964.2017.03.014

Abstract

Abstract:

In order to decipher the mechanism of PPV-VLP presentation by dendritic cells (DC_s), CD172a⁺ CD11R⁺ DC and CD4^- CD8⁺ T cell were isolated from spleen of unvaccinated and CSFV vaccinated pigs, respectively, using magnetic beads. Purified splenic DCs were preincubated for 1 h with primaquin, cycloheximide, chloroquine, lactacystin, brefeldin A, leupeptin, or pepstatin. Then PPV-VLPs-E290 was added, and the DCs were incubated for 4 h in the continued presence of these different drugs. The presentation assay of PPV-VLPs-E290 was monitored by cytotoxic activity of CD8⁺ T cells, which was measured using the Lactate dehydrogenase (LDH) release assay. The results showed that incubation of DCs with PPV-VLPs-E290 in the presence of primaquin and leupetin did not block PPV-VLPs-E290 presentation. However, the present efficiency of DCs pretreated with chloroquin, cycloheximide, brefeldin A and lactacystin was severely reduced. These results showed that DCs can cross-present PPV-VLPs-E290. Both acidification of late endosomes and protease hydrolysis are necessary for PPV-VLPs-E290 processing.

Key words: parvovirus-like particles, dendritic cells, presentation pathways

CLC Number:

S852.659.2

LUO Shang-xing, SHEN Xiao-qiang, GU Wen-yuan, FAN Jing-hui, DI Jing-mei, LIU Bao-jing, DAI Fei, KONG Yuan-yuan, CHANG Yan-yan, ZUO Yu-zhu. Studies on Presentation of Recombinant Parvovirus-like Particles by Porcine Splenic Dendritic Cells[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 508-514.

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[1]	LUO Shang-xing, SHEN Xiao-qiang, GU Wen-yuan, FAN Jing-hui, DI Jing-mei, LIU Bao-jing, DAI Fei, KONG Yuan-yuan, CHANG Yan-yan, ZUO Yu-zhu. Studies on Presentation of Recombinant Parvovirus-like Particles by Porcine Splenic Dendritic Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(3): 508-514.
[2]	SHEN Xiao-qiang，ZUO Yu-zhu，DI Jing-mei，GU Wen-yuan，FAN Jing-hui. The Influence of Antibodies against Key Factors of Different Endocytic Pathways on Dendritic Cells Uptake Recombinant Parvovirus-like Particles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1924-1930.
[3]	SHEN Xiao-qiang，ZUO Yu-zhu，DI Jing-mei，GU Wen-yuan，FAN Jing-hui. The Influence of Antibodies against Key Factors of Different Endocytic Pathways on Dendritic Cells Uptake Recombinant Parvovirus-like Particles [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(9): 1924-1930.
[4]	SHEN Yu-meng，YANG Qian. Effects of Intranasal Administration with Bacillus subtilis Spores on the Dendritic Cells in Porcine Tonsils [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 849-854.
[5]	SHEN Yu-meng，YANG Qian. Effects of Intranasal Administration with Bacillus subtilis Spores on the Dendritic Cells in Porcine Tonsils [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(5): 849-854.
[6]	GAO Yun-huan, LI Na, DONG Chang-hai, TANG Ran-xiao, LI Li-min, WANG Jia-xin. The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 129-135.
[7]	GAO Yun-huan, LI Na, DONG Chang-hai, TANG Ran-xiao, LI Li-min, WANG Jia-xin. The Role of Scavenger Receptors on Dendritic Cells in Presenting VP1-VP4 Antigen of Foot-and-Mouth Disease Virus to T Cells [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(1): 129-135.
[8]	WANG Zhensheng;NIE Xiaohua;LI Huanrong;SUN Yingjian;SUI Lihua;CUI Defeng . Changes of Inflammatory Reactions of Porcine Skin Dendritic Cells Infected with Porcine Circovirus Type 2 in vivo [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(12): 1776-1781.
[9]	WANG Zhensheng;NIE Xiaohua;LI Huanrong;SUN Yingjian;SUI Lihua;CUI Defeng . Changes of Inflammatory Reactions of Porcine Skin Dendritic Cells Infected with Porcine Circovirus Type 2 in vivo [J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2009, 40(12): 1776-1781.

Studies on Presentation of Recombinant Parvovirus-like Particles by Porcine Splenic Dendritic Cells

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