携带NDV HN基因疫苗的重组减毒鸡白痢沙门菌口服免疫原性研究

doi:10.11843/j.issn.0366-6964.2016.10.017

Abstract

Abstract:

To study the oral immunogenicity of attenuated Salmonella pullorum，carried HN (haemagglutinin-neuraminidase) gene vaccine of Newcastle disease virus (NDV).In this study，the recombinant plasmid pcDNA3-HN was finally electro-transformed into attenuated Salmonella pullorum ΔcrpC79-13 to construct the recombinant strain ΔcrpC79-13 (pcDNA3-HN).Then the 7-day-old chickens were oral inoculated with 1×10⁹CFU recombinant strain ΔcrpC79-13 (pcDNA3-HN)，and the Δcrp C79-13 (pcDNA3)，PBS，NDV Ⅳvaccine were act as the control group.Then the dynamic level of HI antibody and intestinal mucosal IgA antibody against NDV were determined at 7，14，21，28，35 days post vaccination.At the same time，the level of peripheral blood lymphocyte proliferation of immune chicken and the challenge protection efficiency were determined.The results showed that the recombinant vaccine strains Δcrp C79-13 (pcDNA3-HN) carrying NDV HN gene vaccine was constructed successfully，which could induce HI antibody against NDV at 14-35 d after immunization and reach the highest value at 21 d after immunization.The content of intestinal mucosal IgA antibody in Δcrp (C79-13 pcDNA3-HN) group was slightly higher than that in Δcrp C79-13 (pcDNA3) group，but the difference was not significant (P> 0.05).The peripheral blood lymphocyte stimulation index of Δcrp (C79-13 pcDNA3-HN) group was significantly higher than that of the PBS control group (P<0.01)，and was slightly higher than that of the ΔcrpC79-13 (pCDNA3) group （P＜0.05) at 28 d after immunization.The immunity protection rate against strong virus was 67%（10/15） in experiment group with 10³EID₅₀NDV F₄₈E₉，the protection rate was 100% which challenged with 10⁸ CFU Salmonella pullorum C79-13 strain.The results showed that the recombinant attenuated Salmonella pullorum ΔcrpC79-13 (pcDNA3-HN) strains have good oral immunogenicity.

Key words: NDV, HN gene, attenuated Salmonella bacteria, oral immunization, immunogenicity

CLC Number:

S852.52

DING Ke，YU Zu-hua，LI Meng，YU Chuan，SHANG Ke，CHENG Xiang-chao，CHEN Gui-hua，LIAO Cheng-shui, JIA Yan-yan, WANG Yang, ZHANG Chun-jie. Oral Immunogenicity of an Attenuated Salmonella pullorum Harbouring HN Gene Vaccine of Newcastle Disease Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(10): 2081-2088.

References

［1］JIANG Y L，HU J T，GUO Y B，et al.Construction and immunological evaluation of recombinant Lactobacillus plantarum expressing HN of Newcastle disease virus and DC-targeting peptide fusion protein［J］.J Biotechnol，2015，216：82-89.
［2］杨煦，刘玉芬，刘怀然.新城疫疫苗的研究进展［J］.现代农业科技，2012（2）：325-327.
YANG X，LIU Y F，LIU H R.Research progresses of Newcastle disease vaccine［J］.Modern Agricultural Science and Technology，2012（2）：325-327.(in Chinese)
［3］MORRISON T G.Structure and function of a paramyxovirus fusion protein［J］.Biochim Biophys Acta，2003，1614(1)：73-84.
［4］侯巍.新城疫病毒分子生物学及其疫苗的研究进展［J］.草业与畜牧，2011（9）：7-10.
HOU W.Study progress in application of molecular biology in Newcastle disease virus and vaccines［J］.Prataculture & Animal Husbandry，2011（9）：7-10.(in Chinese)
［5］GENTSCHEV I，DIETRICH G，SPRENG S，et al.Recombinant attenuated bacteria for the delivery of subunit vaccines［J］.Vaccine，2001，19 ( 17)：2621-2628.
［6］CHIRULLO B，AMMENDOLA S，LEONARDI L，et al.Attenuated mutant strain of Salmonella Typhimurium lacking the ZnuABC transporter contrasts tumor growth promoting anti-cancer immune response［J］.Oncotarget，2015，6(19)：17648-17660.
［7］HAN L，ZHEN Y H，LIANG A X，et al.Oral vaccination with inhibin DNA delivered using attenuated Salmonella choleraesuis for improving reproductive traits in mice［J］.J Basic Microbiol，2014，54(9)：962-968.
［8］张明亮，张春杰，程相朝.沙门氏菌减毒及其作为疫苗活载体的研究进展［J］.生物学杂志，2012(2)：65-68.
ZHANG M L，ZHAGN C J，CHENG X C.Advances in attenuation for salmonella and attenuated salmonella as vaccine carrier［J］.Journal of Biology，2012(2)：65-68.(in Chinese)
［9］YANG X，ZHOU Y S，LI J N，et al.Recombinant infectious bronchitis virus (IBV) H120 vaccine strain expressing the hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) protects chickens against IBV and NDV challenge［J］.Arch Virol，2016，161(5)：1209-1216.
［10］王善辉，薛忠，汪鹏旭，等.昆虫细胞表达基因Ⅶ NDV HN基因的免疫效力研究［J］.安徽农业大学学报，2015，43(1)：1-5.
WANG S H，XUE Z，WANG P X，et al.Expressions of protein HN of Newcastle disease virus in recombinant baculovirus and its antigenicity analysis［J］.Journal of Anhui Agricultural University，2015，43 (1)：1-5.(in Chinese)
［11］潘志明，焦新安，张小荣，等.运送鹅源新城疫病毒DNA疫苗的减毒沙门氏菌及其免疫原性［J］.农业生物技术学报，2004，12(4)：412-415.
PANG Z M，JIAO X A，ZHANG X R，et al.DNA vaccine against Newcastle disease virus of goose origin delivered by attenuated Salmonella typhimurium and its immunogenicity for mice［J］.Journal of Agricultural Biotechnology，2004，12(4)：412-415.(in Chinese)
［12］潘志明，黄金林，程宁宁，等.稳定携带新城疫病毒DNA疫苗减毒沙门氏菌的构建及其免疫原性［J］.病毒学报，2008，24(1)：41-46.
PAN Z M，HUANG J L，CHENG N N，et al.Construction and immunogenicity of attenuated Salmonella typhimurium stably harbouring DNA vaccine against Newcastle disease virus［J］.Chinese Journal of Virology，2008，24(1)：41-46.(in Chinese)
［13］张丽君，罗开健.减毒沙门菌在禽用疫苗应用中的研究进展［J］.动物医学进展，2007，28(8)：73-76.
ZHANG L J，LUO K J.Study progress of attenuated Salmonella in the application of avain vaccines［J］.Progress in Veterinary Medicine，2007，28(8)：73-76.(in Chinese)
［14］HUANG L Y，WANG K Y，XIAO D，et al.Safety and immunogenicity of an oral DNA vaccine encoding Sip of Streptococcus agalactiae from Nile tilapia Oreochromis niloticus delivered by live attenuated Salmonella typhimurium［J］.Fish Shellfish Immunol，2014，38(1)：34-41.
［15］甘军纪，陶鸽，张胜文，等.鸡新城疫重组鸡痘病毒活疫苗的免疫持续期和加强免疫研究［J］.中国兽医学报，2011，12(31)：1681-1685.
GAN J J，TAO G，ZHANG S W，et al.Duration of immunity and boost vaccination of a recombinant fowlpox virus expressing Newcastle disease virus HN gene［J］.Chinese Journal of Veterinary Science，2011，12(31)：1681-1685.(in Chinese)
［16］孙景秀，王桂华，侯喜林，等.锚定NDV HN 蛋白重组干酪乳杆菌诱导雏鸡黏膜免疫应答［J］.黑龙江八一农垦大学学报，2012，24(2)：43-47.
SUN J X，WANG G H，HOU X L，et al.The mucosal immune response induced in chicks immunized with Lactobacillus casei anchoring NDV HN Protein［J］.Journal of Heilongjiang Bayi Agricultural University，2012，24(2)：43-47.(in Chinese)
［17］程相朝，郁川，廖成水，等.减毒鸡白痢沙门氏菌ΔcrpC79-13株的构建及其对雏鸡的免疫活性［J］.中国兽医科学，2012，42(1)：19-25.
CHENG X C，YU C，LIAO C S，et al.Construction of an attenuated Salmonella pullorum Δcrp C79-13 mutant and its immunogenicity in chicks［J］.Chinese Veterinary Science，2012，42(1)：19-25.(in Chinese)
［18］SUN Y，ZHANG Y，SHI C，et al.Newcastle disease virus vaccine encapsulated in biodegradable nanoparticles for mucosal delivery of a human vaccine［J］.Hum Vaccin Immunother，2014，10(8)：2503-2506.
［19］沙万里，宋泰，尹柏双，等.新城疫病毒F-HN基因DNA疫苗的免疫效果评价［J］.吉林农业科技学院学报，2014，23(1)：7-9.
SHA W L，SONG T，YIN B S，et al.Evaluation of immune efficacy with DNA vaccines based on F and HN gene of Newcastle disease virus［J］.Journal of Jilin Agricultural Science and Technology University，2014，23(1)：7-9.(in Chinese)
［20］KUMAR S，NAYAK B，COLLINS P L, et al. Evaluation of the Newcastle disease virus F and HN proteins in protective immunity by using a recombinant avian paramyxovirus type 3 vector in chickens［J］.J Virol，2011，85(13)：6521-6534.

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Oral Immunogenicity of an Attenuated Salmonella pullorum Harbouring HN Gene Vaccine of Newcastle Disease Virus

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