黑曲霉发酵白术对小鼠胸腺指数、脾指数及脾T淋巴细胞增殖的影响

doi:10.11843/j.issn.0366-6964.2016.07.026

Abstract

Abstract:

This study aimed to study the effect of Aspergillus niger-fermented Atractylodes macrocephala Koidz on the immune function of mice.An immunosuppression mice model (cyclophosphamide) was utilize to study the effect of fermented Atractylodes macrocephala Koidz on spleen index，thymus index and T lymphocyte proliferation.High performance liquid chromatography (HPLC) was used to analyze the changes in the active omponents of Atractylodes macrocephala Koidz with or without fermentation by Aspergillus niger.Orthogonal experiment was conducted to select the best conditions.The results showed that：1)The low，middle and high dose groups (0.5，1，2 g•kg^-1) of fermented Atractylodes macrocephala Koidz can effectively increase thymus index (P<0.05).In addition，middle dosage group of fermented Atractylodes macrocephala Koidz can effectively increase spleen index and T lymphocyte proliferation (P<0.05)；2) The results of HPLC showed that the content of atractylenolide Ⅰ was effectively increased in the Atractylodes macrocephala Koidz processed by Aspergillus niger compared with unfermented Atractylodes macrocephala Koidz；3) The best fermentation conditions were as follows：inoculum concentration was 8%；water content was 40%；fermentation time was 96 h，under the optimized conditions，the content of atractylenolide Ⅰ was 1.4 times of those in raw Atractylodes macrocephala Koidz.These results indicated that fermented Atractylodes macrocephala Koidz could improve immune function of mice by increasing the content of atractylenolide Ⅰ.

Key words: Aspergillus niger, fermented Atractylodes macrocephala Koidz, immune function, atractylenolide Ⅰ

CLC Number:

S853.73

SUN Xiang-wan，WANG Yun，WANG Xin，LIU Xiao-ye，MU Xiang，DONG Hong. Effect of Aspergillus niger-Fermented Atractylodes macrocephala Koidz on the Spleen Index，Thymus Index and T Lymphocyte Proliferation of Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1502-1510.

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Effect of Aspergillus niger-Fermented Atractylodes macrocephala Koidz on the Spleen Index，Thymus Index and T Lymphocyte Proliferation of Mice

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