禽呼肠孤病毒σA和σNS蛋白激活PI3K/Akt信号通路的研究

doi:10.11843/j.issn.0366-6964.2016.07.019

Abstract

Abstract:

The aim of the present study was to find out whether ARV activates the phosphatidylinositol 3-Kinase-dependent Akt (PI3K/Akt) pathway according to the PXXP or YXXXM motif of σA and σNS protein.Gene splicing by overlap extension PCR was used to change the PXXP or YXXXM motif of σA and σNS gene.Recombined plasmids that contain mutant σA and σNS genes were generated.The Akt phosphorylation profile of transfected cells were examined by flow cytometry and Western blot.The results showed that σA and σNS genes were expressed in the Vero cells，and the expression of P-Akt of the σA mutant groups (Amino acid 110-114 and 114-117) decreased markedly.The results indicated that the σA protein of ARV activate the PI3K/Akt pathway by the PXXP motif.The results of this study reveal the mechanisms by which ARV manipulate the cellular signal transduction pathways，which may provide new ideas for novel drug targets.

Key words: ARV, PI3K/Akt pathway, σA protein, PXXP motif

CLC Number:

S852.659.4

XIE Li-ji，XIE Zhi-xun，HUANG Li，DENG Xian-wen，XIE Zhi-qin，FAN Qing，LUO Si-si，HUANG Jiao-ling，ZENG Ting-ting. The Study of the Activation of PI3K/Akt Pathway by the σA and σNS Protein of Avian Reovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1451-1458.

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The Study of the Activation of PI3K/Akt Pathway by the σA and σNS Protein of Avian Reovirus

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