WANG Wei-wei，LIU Rui-qi，WU Yong-yan，YANG Yan-ge，WANG Yong-sheng，QING Su-zhu. The Progress of CRISPR/Cas9 System and Its Application in Animal Genetic Engineering[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(7): 1299-1305.
［1］ISHINO Y，SHINAGAWA H，MAKINO K，et al.Nucleotide sequence of the iap gene，responsible for alkaline phosphatase isozyme conversion in Escherichia coli，and identification of the gene product［J］.J Bacteriol，1987，169：5429-5433.
［2］GAJ T，GERSBACH C A，BARBAS C F，et al.ZFN，TALEN，and CRISPR/Cas-based methods for genome engineering［J］. Cell，2013，31：397-405.
［3］MAKAROVA K S，ARAVIND L，GRISHIN N V，et al.A DNA repair system specific for thermophilic Archaea and bacteria predicted by genomic context analysis［J］.Nucleic AcidsRes，2002，30(2)：482-496.
［4］GUY C P，MAJERNÍK A I，CHONG J P，et al.A novel nuclease-ATPase (Nar71) from archaea is part of a proposed thermophilic DNA repair system［J］.Nucleic Acids Res，2004，32(21)：6176-6186.
［5］GRISSA I，VERGNAUD G，POURCEL C，et al.The CRISPRdb database and tools to display CRISPRs and to generate dictionaries of spacers and repeats［J］.BMCBioinformatics，2007，8：172.
［6］HSU P D，LANDER E S，ZHANG F，et al.Development and Applications of CRISPR-Cas9 for Genome engineering［J］.Cell，2014，157：1262-1278.
［7］PELLETIER S，GINGRAS S，GREEN D R，et al.Mouse genome engineering via CRISPR-Cas9 for study of immune function［J］.Immunity，2015，42：18-27.
［8］HSU P D，SCOTT D A，WEINSTEIN J A，et al.DNA targeting specificity of RNA-guided Cas9 nucleases［J］.Nat Biotechnol， 2013，31(9)：827-832.
［9］DOUDNA J A，CHARPENTIER E.The new frontier of genome engineering with CRISPR-Cas9［J］.Science，2014，246：1258096(1-9).
［10］RAN F A，HSU P D，WRIGHT J，et al.Genome engineering using the CRISPR-Cas9 system［J］.Nat Protocols，2013，8：2281-2308.
［11］XIAO A，CHENG Z，KONG L，et al.CasOT：a genome-wide Cas9/gRNA off-target searching tool［J］.Bioinformatics，2014，30(8)：1180-1182.
［12］HOCKEMEYER D，WANG H，KIANI S，et al.Genetic engineering of human pluripotent cells using TALEnucleases［J］.Nat Biotechnol，2011，29(8)：731-734.
［13］CONG L，RAN F A，COX D，et al.Multiplex genome engineering using CRISPR/Cas systems［J］.Science，2013，339：819-823.
［14］SHALEM O，SANJANA N E，HARTENIAN E，et al.Genome-scale CRISPR-Cas9 knockout screening in human cells［J］.Science，2014，343：84-87.
［15］MALI P，YANG L，ESVELT K M，et al.RNA-guided human genome engineering via Cas9［J］.Science，2013，339(6121)：823-826.
［16］DIANOV G L，HÜBSCHER U.Mammalian base excision repair：the forgotten archangel［J］.Nucleic Acids Res， 2013，41(6)：3483-3490.
［17］KIM H，KIM J S.A guide to genome engineering with programmable nucleases［J］.Nat Rev Genet，2014，15(5)：321-334.
［18］方锐，畅飞，孙照霖，等.Crispr/Cas9介导的基因组定点编辑技术［J］.生物化学与生物物理进展，2013，40(8)：691-702.
FANG R，CHANG F，SUN Z L，et al.New method of genome editing derived from CRISPR/Cas9［J］.Progress in Biochemistry and Biophysics，2013，40(8)：691-702.(in Chinese)
［19］GAJ T，GERSBACH C A，BARBAS C F.ZFN，TALEN，and CRISPR/Cas-based methods for genome engineering［J］.Trends Biotechnol，2013，31(7)：397-405.
［20］SAPRANAUSKAS R，GASIUNAS G，FREMAUX C，et al.The Streptococcus thermophilus CRISPR/Cas system provides immunity in Escherichia coli［J］.Nucleic Acids Res，2011，39(21)：9275-9282.
［21］DOW L E，FISHER J，O’ROURKE K P，et al.Inducible in vivo genome editing with CRISPR-Cas9［J］. Nat Biotechnol， 2015，33(4)：390-394.
［22］KLEINSTIVER B P，PREW M S，TSAI S Q，et al.Engineered CRISPR-Cas9 nucleases with altered PAM specificities［J］.Nature，2015，523(7561)：481-485.
［23］JIANG W，BIKARD D，COX D，et al.RNA-guided editing of bacterial genomes using CRISPR-Cas systems［J］. Nat Biotechnol，2013，31(3)：233-239.
［24］CHO S W，KIM S，KIM J M，etal.Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease［J］.Nat Biotechnol，2013，31(3)：230-232.
［25］JINEK M，EAST A，CHENG A，et al.RNA-programmed genome editing in human cells［J］.Elife，2013，2(1)：e00471.
［26］NISHIMASU H，RAN F A，HSU P D，et al.Crystal structure of Cas9 in complex with guide RNA and target DNA［J］.Cell，2014，156(5)：935-949.
［27］CARROLL D.Staying on target with CRISPR-Cas［J］.Nature America，2013，31：807-809.
［28］MONTAGUE T G，CRUZ J M，GAGNON J A，et al.CHOPCHOP：a CRISPR/Cas9 and TALEN web tool for genome editing［J］.Nucleic Acids Res，2014，25：1-7.
［29］WILES M V，QIN W，CHENG A W，et al.CRISPR-Cas9-mediated genome editing and guide RNA design［J］.Mamm Genome，2015，26(9-10)：501-510.
［30］GUSMAO E G，DIETERICH C，ZENKE M，et al.Detection of active transcription factor binding sites with the combination of DNase hypersensitivity and histone modifications［J］.Bioinformatics，2014，30(22)：3143-3151.
［31］RAN F A，HSU P D，LIN C Y，et al.Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity［J］.Cell，2013，154(6)：1380-1389.
［32］BHAKTA M S，SEGAL D J.The generation of zinc finger proteins by modular assembly［J］.Methods Mol Biol，2010，649：3-30.
［33］KIM D，BAE S，PARK J，et al.Digenome-seq：genome-wide profiling of CRISPR-Cas9 off-target effects in human cells［J］.Nat Methods，2015，12(3)：237-243.
［34］CHO S W，KIM S，KIM Y，et al.Analysis of off-target effects of CRISPR/Cas-derived RNA-guided endonucleases and nickases［J］. Genome Res，2014，24(1)：132-141.
［35］FU Y F，SANDER J D，REYON D，et al.Improving CRISPR-Cas nuclease specificity using truncated guide RNAs［J］.Nat Biotechnol，2014，32(3)：279-284.
［36］TREVINO A E，ZHANG F.Chapter eight-genome editing using Cas9 nickases［J］.Methods Enzymol，2014，546：161-174.
［37］YANG H，WANG H，SHIVALILA C S，et al.One-step generation of mice carrying reporter and conditional alleles by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，153：1370-1379.
［38］WANG H，YANG H，SHIVALILA C S，et al.One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering［J］.Cell，2013，153：910-918.
［39］NIU Y，SHEN B，CUI Y，et al.Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos［J］.Cell，2014，156：836-843.
［40］CHEN F，WANG Y，YUAN Y，et al.Generation of B cell-deficient pigs by highly efficient CRISPR/Cas9-mediated gene targeting［J］.J Genet Genomics，2015，42(8)：437-444.
［41］TU Z，YANG W，YAN S，et al.CRISPR/Cas9：a powerful genetic engineering tool for establishing large animal models of neurodegenerative diseases［J］.Mol Neurodegener，2015，10：35-37.
［42］ZHOU X，XIN J，FAN N，et al.Generation of CRISPR/Cas9-mediated gene-targeted pigs via somatic cell nuclear transfer［J］.Cell Mol Life Sci，2015，72(6)：1175-1184.
［43］CRISPO M，MULET A P，TESSON L，et al.Efficient generation of myostatin knock-out sheep using CRISPR/Cas9 technology and microinjection into zygotes［J］.PLoS One，2015，10(8)：366-375.
［44］WANG X，YU H，LEI A，et al.Generation of gene-modified goats targeting MSTN and FGF5 via zygote injection of CRISPR/Cas9 system［J］.Sci Rep，2015，5：13878.
［45］YAN Q，ZHANG Q，YANG H，et al.Generation of multi-gene knockout rabbits using the Cas9/gRNA system［J］.Cell Regen(Lond)，2014，3(1)：12.
［46］VÉRON N，QU Z，KIPEN P A，et al.CRISPR mediated somatic cell genome engineering in the chicken［J］.Dev Biol，2015，407(1)：68-74.
［47］KOTANI H，TAIMATSU K，OHGA R，et al.Efficient multiple genome modifications induced by the crRNAs，tracrRNA and Cas9 protein complex in zebrafish［J］.PLoS One，2015，10(5)：128-134.
［48］HRUSCHA A，KRAWITZ P，RECHENBERG A，et al.Efficient CRISPR/Cas9 genome editing with low off-target effects in zebrafish［J］.Development，2013，140(24)：4982-4987.
［49］WANG Z.Genome engineering in cattle：recent technological advancements［J］.Chromosome Res，2015，23(1)：17-29.
［50］HE X，TAN C，WANG F，et al.Knock-in of large reporter genes in human cells via CRISPR/Cas9-induced homology-dependent and independent DNA repair［J］.Nucleic AcidsRes，2016，［Epub ahead of print］.
［51］ZETSCHE B，GOOTENBERG J S，ABUDAYYEH O O，et al.Cpfl is a single RNA-guided endonuclease of a class 2 CRISPR-Cas system［J］.Cell，2015，163(3)：759-771.
［52］SLAYMAKER I M，GAO L，ZETSCHE B，et al.Rationally engineered Cas9 nucleases with improved specificity［J］.Science，2016，351(6268)：84-88.
［53］JIANG W，ZHOU H，BI H，et al.Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis，tobacco，sorghum and rice［J］.Nucleic AcidsRes， 2013，41(20)：188-193.
