Construction and Packaging of Recombinant Lentivirus Interference Vector Specific to Porcine MyD88 Gene and the Evaluation of the Corresponding Lentivirus
ZI Chen1,XIA Ri-wei1,YIN Xue-mei1,YU Li-huai1,ZHU Guo-qiang2,WU Sheng-long1* ,BAO Wen-bin1*
(1.Animal Science and Technology College,Yangzhou University,Key Laboratory for Animal Genetics, Breeding,Reproduction and Molecular Design of Jiangsu Province,Yangzhou 225009,China;2.College of Veterinary Medicine,Yangzhou University,Yangzhou 225009,China)
ZI Chen,XIA Ri-wei,YIN Xue-mei,YU Li-huai,ZHU Guo-qiang,WU Sheng-long,BAO Wen-bin. Construction and Packaging of Recombinant Lentivirus Interference Vector Specific to Porcine MyD88 Gene and the Evaluation of the Corresponding Lentivirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(4): 657-664.
[1]MUZIO M,POLENTARUTTI N,BOSISIO D,et al.Toll-like receptor family and signalling pathway[J].Biochem Soc Trans,2000,28(5):563-566.
[2]GHOSH S,KARIN M.Missing pieces in the NF-kappaB puzzle[J].Cell,2002,109(Suppl):S81-S96.
[3]JANSSENS S,BEYAERT R.Functional diversity and regulation of different interleukin-1 receptor-associated kinase (IRAK) family members[J].Mol Cell,2003,11(2):293-302.
[4]O′NEILL L A.Signal transduction pathways activated by the IL-1 receptor/toll-like receptor superfamily[J].Curr Top Microbiol Immunol,2002,270:47-61.
[5]SUZUKI N,SUZUKI S,YEH W C.IRAK-4 as the central TIR signaling mediator in innate immunity[J].Trends Immunol,2002,23(10):503-506.
[6]SUZUKI N,SUZUKI S,DUNCAN G S,et al.Severe impairment of interleukin-1 and Toll-like receptor signalling in mice lacking IRAK-4[J]. Nature,2002,416(6882):750-756.
[7]LI X,LIU H,YANG S,et al.Characterization analysis and polymorphism detection of the porcine MyD88 gene[J].Genet Mol Biol,2009,32(2):295-300.
[8]TOHNO M,SHIMAZU T,ASO H,et al.Molecular cloning and functional characterization of porcine MyD88 essential for TLR signaling[J].Cell Mol Immunol,2007,4(5):369-376.
[9]MOSES T,WAGNER L,FLEMING S D.TLR4-mediated Cox-2 expression increases intestinal ischemia/reperfusion-induced damage[J]. J Leukoc Biol,2009,86(4):971-980.
[10]LOIARRO M,CAPOLUNGHI F,FANTÒ N,et al.Pivotal advance:Inhibition of MyD88 dimerization and recruitment of IRAK1 and IRAK4 by a novel peptidomimetic compound[J].J Leukoc Biol,2007,82(4):801-810.
[11]POLTORAK A,HE X,SMIRNOVA I,et al.Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice:mutations in Tlr4 gene[J].Science,1998,282(5396):2085-2088.
[12]KAWAI T,ADACHI O,OGAWA T,et al.Unresponsiveness of MyD88-deficient mice to endotoxin[J].Immunity,1999,11(1):115-122.
[13]TAUSZIG-DELAMASURE S,BILAK H,CAPOVILLA M,et al.Drosophila MyD88 is required for the response to fungal and Gram-positive bacterial infections[J].Nat Immunol,2002,3(1):91-97.
[14]YAMAWAKI Y,KIMURA H,HOSOI T,et al.MyD88 plays a key role in LPS-induced Stat3 activation in the hypothalamus[J]. Am J Physiol Regul Integr Comp Physiol,2010,298(2):R403-410.
[15]DROUIN-OUELLET J,LEBEL M,FILALI M,et al.MyD88 deficiency results in both cognitive and motor impairments in mice[J].Brain Behav Immun,2012,26(6):880-885.
[16]罗庆苗,苗向阳,张瑞杰.转基因动物新技术研究进展[J].遗传,2011,33(5):449-458.
LUO Q M,MIAO X Y,ZHANG R J.An update on the development of transgenic animal technology[J].Hereditas (Beijing),2011,33(5):449-458.(in Chinese)
[17]杨浩,李新建,程佳,等.慢病毒介导shRNA干扰颗粒体蛋白前体(GRN)促进猪前体脂肪细胞分化[J].农业生物技术学报,2012,20(6):599-605.
YANG H,LI X J,CHENG J,et al.Lentivirus mediated granulin shRNA interference silencing promotes pig preadipocyte differentiation[J].Journal of Agricultural Biotechnology,2012,20(6):599-605.(in Chinese)
[18]程佳,蒲蕾,杨浩,等.慢病毒介导shRNA干扰RBP4促进猪前体脂肪细胞成脂分化[J].畜牧兽医学报,2013,44(6):937-943.
CHENG J,PU L,YANG H,et al.Lentivirus mediated shRNA interference silencing RBP4 promoted differentiation of porcine preadipocytes[J].Acta Veterinaria et Zootechnica Sinica,2013,44(6):937-943.(in Chinese)
[19]LAN Y,ZHAO K,HE W,et al.Inhibition of porcine hemagglutinating encephalomyelitis virus replication by short hairpin RNAs targeting of the nucleocapsid gene in a porcine kidney cell line[J].J Virol Methods,2012,179(2):414-418.
[20]BAVYKIN A S,MISHIN D V,KARPUKHIN A V,et al.The ability of small interfering RNA oligonucleotides to decrease the infective activity of hepatitis C virus in the cell cultures[J].Vopr Virusol,2012,57(2):32-34.
[21]周谦君,范慧敏,刘中民.MyD88siRNA诱导猪调节性树突状细胞的实验研究[J].中国细胞生物学学报,2010,32(3):415-421.
ZHOU Q J,FAN H M,LIU Z M.MyD88 knockdown by liposome-mediated RNA interference to porcine monocyte-derived dendritic cell[J].Chinese Journal of Cell Biology,2010,32(3):415-421.(in Chinese)