马立克病病毒感染鸡心组织肿瘤病变与热休克蛋白表达的相关性分析

doi:10.11843/j.issn.0366-6964.2014.12.015

Abstract

Abstract:

The tumor model of Marek’s disease (MD) was established by means of artificial MDV infection.Relationship between pathological lesion of heart and distribution，expression level of heat shock proteins (HSPs) were studied during the progress of tumor.At the day 7，14，21，28，35，42 and 86 post artificial infection (PI)，chickens were killed and the hearts were sampled.Pathological lesion of heart and distribution，expression level of HSPs were studied during the progress of tumor by the methods of histopathology，immunohistochemistry and ELISA.Results were as follows：At 14 days of age，obvious pathological damage appeared in the hearts of chickens attacked by MDV，and unequal-sized lymphoid cells infiltration was found among myocardial fiber.HSP90 was mainly located in the cytoplasm of tumor cell and HSP70 was mainly located in the cytoplasm of myocardial fiber.Within the course of MD，the content of HSP90 of infected group was always higher than that of blank control group and vaccine control group，which was significantly higher after 28 day’s，and were 4.021 and 3.746 times higher than the control group at the age of 86 days.The content of HSP70 was induced at the age of 35 days.There were no significant difference of HSP90 and HSP70 content between the chickens in the blank control and vaccine control group in the test process.HSP90 was mainly distributed in the cytoplasm of tumor cell and the content of HSP90 was significantly induced in the test process.HSP90 might be a marker to determine the process of tumor caused by MDV.

Key words: Marek’s disease, heat shock protein, histopathology, immunohistochemistry

CLC Number:

PEI Lan-ying，FENG Xiu-juan，LI Juan，LI Yu-bao，WANG Shou-rong，ZHU Yu-wei，WU Wei-sheng. Relationship between Expression Level of HSPs and Heart Tissue Pathological Lesion of Chickens in Progress of Marek’s Disease[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 2013-2018.

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Relationship between Expression Level of HSPs and Heart Tissue Pathological Lesion of Chickens in Progress of Marek’s Disease

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