猪瘟病毒C蛋白与宿主β-actin相互作用的鉴定

doi:10.11843/j.issn.0366-6964.2014.12.012

Abstract

Abstract:

To study the interactions between the classical swine fever virus (CSFV) and the host，coimmunoprecipitation (Co-IP) and subsequent mass spectrometry were used to screen and identify the host proteins interacted with CSFV C protein from PK-15 cells.β-actin was identified as a potential interacting partner of C protein.In addition，the interaction of the two proteins was further confirmed by endogenous Co-IP and GST pulldown.Moreover，laser confocal imaging confirmed that β-actin colocalized with the CSFV C protein in the cytoplasm.These results showed that CSFV C interacted with β-actin which would facilitate further study of interactions between CSFV proteins and host cell proteins.

Key words: classical swine fever virus, capsid protein, β-actin, virus-host interaction

CLC Number:

S852.651

DONG Hong，LI Dan，CHEN Jia-ning，LI Su，HE Wen-rui，FENG Shuo，HE Fan，LIAO Ya-jin，SUN Yuan，HU Yong-hao. Identification of the Interaction between Classical Swine Fever Virus C Protein and Porcine β-actin[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(12): 1995-1999.

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Identification of the Interaction between Classical Swine Fever Virus C Protein and Porcine β-actin

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