羊驼TGF-β1基因的克隆及表达分析

doi:10.11843/j.issn.0366-6964.2014.06.005

Abstract

Abstract:

In order to explore the biological functions of TGF-β1 in coat color formation in alpaca,the experiment was conducted to clone alpaca TGF-β1 gene，investigate the expression of TGF-β1 in different tissues/organs，and examine the relationship between the TGF-β1 expression and alpaca coat color.The TGF-β1 cDNA was cloned from alpaca by 3′ and 5′ RACE.The sequence analysis was performed by DNAman，PROSITE and megAlign.The expression levels of TGF-β1 gene in different organs/tissues were analyzed by qRT-PCR.The full length of TGF-β1 cDNA in alpaca was 1 364 bp (GenBank Accession No.KF887499) with 1 173 bp ORF coding 390 amino acids.Sequence analysis showed alpaca TGF-β1 has typical structures of TGF-β1 family，showing high similarity with responsed proteins from other mammals.Phylogenetic analysis showed that alpaca TGF-β1 gene is much closed to wild boar(Sus scrofa).qRT-PCR showed that the relative gene expression level of TGF-β1 in brown alpaca was 3.5 times than that in white alpaca.TGF-β1 gene was expressed widely in tested tissues in goat while the expression levels were different in those tissues and highest in lymphoid tissue.Cloning and sequence characterization of alpaca TGF-β1 gene plus the expression analysis in different tisssues/organs indicate that TGF-β1 may play important role in alpaca coat color formation，provide the related DNA elements and experimental basic for further investigating on the molecular mechanism of TGF-β1 functions in animal coat color formation and regulation.

Key words: alpaca, TGF-β1 gene, rapid amplification of cDNA ends (RACE), qRT-PCR, expression analysis

CLC Number:

S813.3

JIA Xiao-yun，JIN Lei-hao，DING Na，LUO Dong-ping，FAN Rui-wen，DONG Chang-sheng. Cloning and Expression of TGF-β1 Gene from Alpaca (Lama pacos)[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(6): 885-892.

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Cloning and Expression of TGF-β1 Gene from Alpaca (Lama pacos)

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