ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (3): 410-415.

• 预防兽医 • Previous Articles     Next Articles

Expression of Main Epitope Domains of CD151 from Marc145 Cells and Relationship with Infection of Porcine Reproductive and Respiratory Syndrome Virus

XIAO Yihong1, WANG Weiwei1, ZHANG Lu1, GAO Jiming1, MA Xiaochun1, ZHOU Enmin2*   

  1. 1. College of Veterinary Medicine, Shandong Agricultural University, Taian 271018, China; 2. Immunology Institute of Northwest A & F University, College of Veterinary Medicine, Northwest A & F University, Yangling 712100, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-03-28 Published:2012-03-28
  • Contact: ZHOU Enmin

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Abstract: To study the role of CD151 in porcine reproductive and respiratory syndrome virus (PRRSV) infection, primers specific to CD151 were designed according to the similar gene sequences submitted in GenBank. Total RNA was extracted from Marc145 cell and a 294 bp of CD151 was amplified by RTPCR. The target gene was cloned into pGEX4T3 vector, then was transformed into E. coli and expressed with IPTG inducer. SDSPAGE and Western blot were employed to identify the expressed protein. The protein was purified and used to immunize mice. The antiserum titres were determined by the ELISA and IFA, and were used to block PRRSV infection of Marc145 cells. The results showed that CD151 gene was amplified and cloned into pGEX4T3CD151 vector. The recombinant CD151 protein with MW of 37 kDa was produced. The antiserum reacted specifically with the recombinant protein and blocked PRRSV infection of Marc145 cells. These results provide useful information for further understanding the role of CD151 in PRRSV infection.

Key words: CD151, Marc145 cell, PRRSV, blocking