ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (8): 1281-1286.

• 预防兽医 • Previous Articles     Next Articles

Screening of Antigen Mimotopes of Rabbit Hemorrhagic Disease Virus by Phage Display Technology

YANG Tingya 1, 2, WANG Fang 1*, JIANG Ping 2, HU Bo 1, FAN Zhiyu 1, WEI Houjun 1, XUE Jiabin 1

  

  1. 1. Key Laboratory of Veterinary Biologicals Engineering and Technology of Ministry of Agriculture, National Center for Engineering Research of Veterinary Bioproducts, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China；2. College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2012-02-08 Revised:1900-01-01 Online:2012-08-24 Published:2012-08-24
  • Contact: WANG Fang

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Abstract: The monoclonal antibody (McAb) A3c against rabbit hemorrhagic disease virus had been used as solidphase selective molecule to screen the epitope of RHDV by phage display technology. McAb A3c was coated on the solidphase and then three rounds of biopanning were carried out; 25 phages were selected and amplified to be identified by ELISA, and the positives were sequenced; the serum of mice immunized three times was prepared to determine the immunogenicity of the epitope. The results showed that the specific phages were enriched effectively after three rounds of biopanning; 19 of 25 phages were positive. The sequence analysis of the positive clones showed that highly homogenous sequence (GTDDMDPGTTAA) comparing to antigen was got, which is the antigen mimotopes of RHDV. The sequence DXXDP was the core amino acids in the epitope. In addition, the serum of mice reacted with antigen well and so did positive phages with hyperimmune serum of rabbit against RHDV, which indicated that the epotipe had good immunogenicity and reactionogenicity. This study provide theoretical basis for studying the epitope of RHDV and new vaccines.

Key words: rabbit hemorrhagic disease virus, monoclonal antibody, phage display technology, mimotopes