ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (11): 1632-1637.

• 研究简报 • Previous Articles     Next Articles

Analysis on Polymorphism and Expression in Different Coat Color Skin of Syntaxin17(STX17) Gene in Mongolian Horse

LI Bei1, HE Xiaolong2, MANG Lai3*   

  1. 1. College of Animal Science, Inner Mongolia Agricultural University,Huhhot 010018, China; 2. Inner Mongolia Academy of Agriculture and Animal Science,Huhhot 010031, China; 3.College of Life Science, Inner Mongolia Agricultural University,Huhhot 010018, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
  • Contact: MANG Lai

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Abstract: In order to research the relationship between syntaxin17(STX17) gene and Mongolian horse coat color and mechanism, the polymorphism in intron 6 and the relative expression quantity in different colors skin of Mongolian horse were analyzed by seminested PCR and realtime quantitative PCR. The results showed that 4.6 kb duplication segments existed in intron 6 of STX17 gene in gray Mongolian horse, but not existed in nongray Mongolian horse by seminested PCR. The expression level of STX17 gene was highest in gray skin and lowest in bay skin by realtime quantitative PCR. The results indicate that the STX17 gene has typical relationship with gray Mongolian horse.

Key words: Mongolian horse, STX17 gene, polymorphism, relative expression quantity