ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (1): 48-55.

• 预防兽医 • Previous Articles     Next Articles

The Analysis of Antiviral Cytokines Transcriptional Profiles of PK-15 Cell Cultures Following Infection with Porcine Parvovirus

LI Hou-wei1 , WEI Zhan-yong2, YIN Hai-yan3, CHEN Hong-ying2, LI Jin-lei1,
HAN Zhi-tao1, CUI Bao-an1*   

  1. 1.Key Laboratory for Animal-derived Food Safety of Henan Province, Zhengzhou 450002, China;2.College of Animal Science and Veterinary Medicine, Henan Argricultural University, Zhengzhou 450002, China; 3.Agricultural Quality Standards and Testing Technology Research Center, Henan Academy of Agricultural Sciences， Zhengzhou 450002, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-25 Published:2011-01-25

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Abstract: In order to survey the host inflammatory responses, particularly the antiviral cytokines responses of Porcine Parvovirus (PPV) infection, and to investigate the host-PPV interaction，Porcine Kidney-15 cells (PK-15) were infected by PPV. Then the viral DNA was measured and analyzed using real-time PCR. The transcript level of cytokines (IFN-β, IFN-γ, IFNAR-1, IFNAR-2, MHC-Ⅰ, MHC-Ⅱ, MX1, NOS, 2-5AS, RNase L and IRF-3) were detected by real-time PCR too. The results showed that PPV proliferated rapidly in PK-15 cell 12 hours after infection, and reached peak 48 hours after infection; the transcription levels of IFN-β, IFN-γ, IFNAR-1, IFNAR-2, MHC-Ⅰ, MHC-Ⅱ, MX1, iNOS, 2-5AS, RNase L and IRF-3 were increased obviously, and the transcription level of Mx1 gene was increased 8 423 times at 24 hours after infection. The results revealed that the level of antiviral cytokines increased in PPV infected PK-15 cell.

Key words: porcine parvovirus, cytokines, PK15 cell, transcriptional profiles