Abstract: In order to improve the development efficiency of somatic cell nuclear transfer embryos of Yanbian Yellow cattle, free radical absorptive antioxidants, such as vitamin E, EGCG and sodium selenite, were added in culture medium in vitro with different concentrations for observing the development of the reconstructed embroys. Oocytes were cultured 2022 h in maturation medium, oocytes with first polar body were chose for enucleation, injection, integration and activation, then restructured embryos were cultured in in vitro culture mediums with antioxidants with different concentrations. On the cleavage rate, in VE study, results showed that 100 μmol·L-1 group was significantly higher than those of other concertrations（84.58% vs 57.64%,70.87%,67.64%）;there were no significant differences between 50 and 200 μmol·L-1 groups,but they were both significantly higher than that of 0 μmol·L-1 group（70.87%,67.64% vs 57.64%）;In EGCG study,results showed that 7.5 and 15 μmol·L-1 groups were significantly higher than that of 0 μmol·L-1 group（79.47%,81.67%vs 69.47%）and there were no significant differences between other groups;In SS study,5 and 0 ng·mL-1 group were significantly higher than 10 ng·mL-1 group（80.44% vs 70.27%,67.16%）while there were no significant differences with 2.5 ng·mL-1 group（80.44% vs 73.92%）, and there were no significant differences between other groups.On the Blastocyst rate,in VE study,results showed that 100 μmol·L-1 group was significantly higher than those of other concertrations（26.36% vs 13.04%,18.08%,17.26%）;In EGCG study,results showed that 15 μmol·L-1 group was significantly higher than 0 and 30 μmol·L-1 groups (24.86% vs 8.99%,12.10%）and there was no significant differences with 7.5 μmol·L-1 group;In SS study, 5 ng·mL-1 group was significantly higher than that of 0 and 10 ng·mL-1 groups（23.31% vs 10.32%,12.65%）;2.5 ng·mL-1 group was significantly higher than that of 0 and 10 ng·mL-1 groups（19.97% vs 10.32%,12.65%） while there were no significant differences between other two groups. Adding 100 μmol·L-1 vitamin E,15 μmol·L-1 EGCG or 5 ng·mL-1 sodium selenite alone in vitro into culture medium can enhance the early development rate of Yanbian Yellow cattle restructured embryos in vitro.

Key words: Yanbian Yellow cattle, somatic cell clone, antioxidant