ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2008, Vol. 39 ›› Issue (12): 1753-1758.

• 基础兽医 • Previous Articles     Next Articles

Preliminary Studies on Prokaryotic Expression,Refolding of Duck IFN-α Mature Peptide and Its Activity against Virus

GONG Yong-qiang1,CHENG An-chun1,2*, WANG Ming-shu1,2*,
YANG Mei1, ZHANG Yao2,CHEN Bin1,3,ZHONG Xiao-rong1   

  1. 1.Avain Disease Research Center of Sichuan Agricultural University, Ya’an 625014,China; 2.Key Laboratory of Animal Disease and Human Health of Sichuan Province, Ya’an 625014,China; 3.Sichuan Provincial Animal Epidemic Prevention & Supervision General Office，Chengdu 610041,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-12-24 Published:2008-12-24

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Abstract: In order to obtain DuIFN-α and investigate its biological function, duck interferon-α(DuIFN-α) mature protein gene was cloned in to pMD18-T vector and inserted into the expressing plasmid/ pET32a+. Then,this recombinant plasmid was transformed into engineering bacteria/ BL2l(DE3) and a 37 kD protein was expressed in the form of inclusion bodies after 5 hours induction by 0.4 mmol/L IPTG. The expressed product which was purified and refolded by metal immobilization affinity chromatography(MIAC) have an ideal activity against virus. Its activity of anti-VSV in DEF was up to 12 800 U/mg and DuIFN-α at 15 U/mL can reduce duck plague virus replication by 80% as measured by FQ-PCR.

Key words: duck IFN-α, mature peptide gene, prokaryotic expression, purification refolding by chromatography, activity against virus