Abstract: In this study, out membrane proteins of Bordetella avium strains P5 and P8 were prepared by improved Wooldridge method. The contents of Bordetella avium OMP were determined by Bradford method. SDS-PAGE electrophoresis was performed to identify Bordetella avium OMP. Prepared OMP immunogen was immunized to rabbits for preparation of high titer immuno serum. And then detections of indirect ELISA and indirect immunofluorescence were established by optimization and screening of conditions. The result showed that the contents of Bordetella avium OMP was 320 μg/mL. The protein molecular weight of OMP are 58，47，41，36，24 kDa in P5, and are 58,47,41,38,21,16 kDa in P8. The detection technique of I-ELISA and IFA were simple, rapid and specific. They can be used in study of epidemiology and serology of Bordetella avium. The two methods laid a foundation for fast diagnosis of Bordetella avium.

Key words: Bordetella avium, outer membrane protein, I-ELISA, IFA