转人乳铁蛋白基因山羊乳腺上皮细胞的研究

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2006, Vol. 37 ›› Issue (12): 1282-1286.

• 遗传繁育 • Previous Articles Next Articles

Study on the hLF Gene Transgenic Goat Mammary Epithelial Cells

Received:1900-01-01 Revised:1900-01-01 Online:2006-12-25 Published:2006-12-25

Abstract

Abstract: The recombinant plasmid containing human lactoferrin gene cDNA was transfected into goat mammary epithelial cells after liposome transinfection. Positive cells were selected by G418 and PCR. Positive cells were proliferated and induced by hormone. The result of Western blotting analysis on cultured cell supernatant showed that the transfected cells could express and secrete hLF protein, whose MW was 58 ku.

Key words: human lactoferrin gene mammary glandspecific expressional vector, EGFP, transgenic goat mammary epithelial cells

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Study on the hLF Gene Transgenic Goat Mammary Epithelial Cells

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