Abstract: In this study using the transgenic (GFP) fibroblasts of Saanen dairy goat as donor cells and using the abattoirderived oocytes of normal goat as recipient cells the nuclear transfer experiments had been processed. During experiments the optimization of electricfusion experiment parameters of transgenic nuclear transfer embryos had been done. For the treatment of transgenic reconstruction embryos of Saanen dairy goat, the electricfusion parameter of group B in experiment plans (1.5kV/cm for 30 μs and pulse interval for 1s) is most suitable for the transgenic reconstruction embryos of Saanen dairy goat, the electricfusion rate of 89.4% for cell-cytoplast complexes and the cleavage rate of 68.0% for reconstructed embryos have been obtained.

Key words: transgenic, nuclear transfer, electrofusion, reconstruction embryo, somatic cloning