ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (8): 1222-1229.doi:

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Effects of Ecadherin Antibody on Mouse Early Embryo Development

WU Xinglong 1, WANG Mianjuan 2, HU Chunchao 1, WANG Pengbo 1, LI Xiangyun 1*
   

  1. 1. College of Animal Science and Technology, Agricultural University of Hebei, Baoding 071000, China; 2. Hospital of Agricultural University of Hebei, Baoding 071000, China
  • Received:2011-08-15 Revised:1900-01-01 Online:2012-08-24 Published:2012-08-24
  • Contact: LI Xiangyun

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Abstract: To investigate the effects of Ecadherin antibody (ECCD1) on mouse embryo development, ECCD1 antibody with different concentrations was added into KSOM media when mouse 8cell embryos were cultured in vitro, and then the developmental potential of ECCD1treated embryos were detected by morphology, alkaline phosphatase activity, pluripotent marker (SSEA1, Oct4, Sox2, Nanog) immunostaining, attachment culture in vitro, implantation in vivo, and chimera production. The result showed that ECCD1 significantly delayed embryo compaction and blastocoele formation. ECCD1treated embryos had obvious inner cell mass, positive alkaline phosphatase and pluripotent markers, normal attachment growth in vitro and implantation in vivo. In addition, the chimera production was also improved when 8cell embryos injected by embryonic stem cells were treated with ECCD1. The results indicated that compaction delay by ECCD1 antibody did not affect embryo development potential.