ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (7): 1170-1176.doi:

• 研究简报 • Previous Articles    

Expression Analysis of microRNA155 in Skeletal Muscle at Different Development Stages in Landrace and Tongcheng Pigs

ZHAO Shuanping1,2, ZAN Linsen1*, TANG Zhonglin 2*, LI Kui2
   

  1. 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China; 2. Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-27 Published:2012-07-27
  • Contact: ZAN Linsen*, TANG Zhonglin

PDF (PC)

862

Abstract:

The aim of this study was to detect the expression of miR155 in skeletal muscle development in Landrace (leantype) and Tongcheng (obesetype) pigs, and explore the roles of miR155 in muscle development. Based on the skeletal muscle tissues of 22 stages (12 prenatal and 10 postnatal stages) in Landrace and Tongcheng pigs, respectively, stemloop RTPCR was performed to detect the miR155 expression. Furthermore, the expression of miR155 in various tissues of adult Tongcheng pigs was detected. The miR155 differential expression in skeletal muscle development exhibited different expression patterns between Landrace and Tongcheng pigs. In adult Tongcheng pigs, miR155 was abundantly expressed in small intestines and lung, moderately in heart and placenta, and weakly in other collected tissues. The result indicate that miR155 may be involved in myofibre proliferation and differentiation and further contribute to skeletal muscle development and muscle phenotype with different genotypes.