ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (7): 1163-1169.doi:

• 研究简报 • Previous Articles     Next Articles

Sexpreselected Piglets Derived from Sexed Sperm by Flow Cytometry Sorting

ZENG Youquan 1,2, LU Yangqing 1, YANG Xiaogan 1,

LU Shengsheng1, ZHANG Ming 1, LU Kehuan 1*

  

  1. 1. State Key Laboratory for Conservation and Utilization of Agricultural Bioresource in the Subtropics, Animal Reproduction Institute, Guangxi University, Nanning 530004, China; 2. Southwest University, Chongqing 402460, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-27 Published:2012-07-27
  • Contact: LU Kehuan

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Abstract:

The main objective of this study was to evaluate the efficiency of surgically artificial insemination (XY sperms were srperated by flow cytometry, and then the prevision sex piglets were breeded by artificial insemination) by using the sexed sperm sorted by flow cytometry to produce sexpreselected piglet. The effect of the sorting procedure on nucleic acid content of sperm was analyzed by acridine orange (AO) staining. Three recipient sows were inseminated with sorted Xsperm and were all pregnant and gave birth to a total of 12 piglets, from which 11 were females representing a 91.67% sexing accuracy, while 40% of piglets were female in the control group that inseminated with unsexed sperm. One sow inseminated with sorted Ysperm farrowed 6 male piglets (100%, 6/6) ,while 57.14% of male piglets in the control group. The total of sexing accuracy in this study was 94.4% (17/18) and no abnormality was observed in piglets derived from sexed sperm comparing to those derived from unsorted sperm. There was also no significant difference in pregnancy and farrowing rates between sows inseminated with sexed and unsexed sperm, but the litter size of sows inseminated with sexed sperm were lower than that of sows inseminated with unsexed sperm. There was no significant difference in the nucleic acid content between unsorted sperm and bulk sorted sperm (P＞0.05). Results of this study indicate the feasibility of piglet production from sperm sorted by flow cytometry.