Abstract:

Cataract, which severely affects the vision and has high incidence, is one of the common ophthalmic diseases on canine. Recent research showed that many pathogenesis gave rise to cataract formation, among which oxidative damage was an important factor. However, there was little research focused on the establishment of cataract models on canine. The aim of our study was to establish senile cataract models, which could lay a foundation for the further research. This research picked up 10 healthy dogs, randomly selected 6 dogs as model group and the rest as control group. We used Fenton solution to establish the model and judged it success or not from the following methods: checked the lens with slit lamp microscope, observed the structures of lens by light microscope and TEM, and determined the changes of biochemical indicators. Compared with the control group, the lens cloudy degree of model group changed remarkably, apoptosis phenomenon could be observed with light microscope and TEM in model group, the TSOD, GSHPx and MDA values in model group were significantly different (P<0.05). The results proved that the senile model can be successfully built through intracystic injection with fenton solution.