ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (7): 1103-1110.doi:

• 预防兽医 • Previous Articles     Next Articles

Sequence Analysis of Fourteen Gallibacterium Isolates from Henan and Shanxi Provinces Based on gyrB, 16S rRNA and rpoB Genes

HUANGHFU Heping1，2, YANG Xia1, ZHAO Jun1, WANG Chuanqing1*, CHANG Hongtao1, CHEN Lu1, WANG Xinwei1, LI Qiaojing1, LIU Hongying1

  

  1. 1. Institute of Poultry Diseases, Henan Agricultural University, Zhengzhou 450002, China; 2. Zhengzhou College of Animal Husbandry Engineering, Zhengzhou 450011, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-07-27 Published:2012-07-27
  • Contact: WANG Chuanqing

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Abstract:

The objective of this study was to analyze the evolutionary relationships of the fourteen Gallibacterium strains isolated from Henan and Shanxi Provinces, and the role of gyrB gene in phylogenesis analysis. 16S rRNA, rpoB and gyrB gene of all isolates were amplified by PCR and the purified PCR product were submitted directly for sequencing. Sequences of three housekeeping genes of 14 Gallibacterium isolates were compared, and used to generate the phylogenetic tree, with that of the Gallibacterium reference strains, using the software of DNAstar, Clustalx 1.81 and Phylip 3.67. The sequence homology were 96.3%98.0% (gyrB), 97.7%99.6% (16S rRNA), 97.7%99.0% (rpoB) among Gallibacterium isolates and G. anatis type strains, 88.8%89.9% (gyrB), 96.2%97.5% (16S rRNA), 92.6%93.6% (rpoB) among Gallibacterium isolates and Gallibacterium genomosp. 1 strains. Three phylogenetic trees generated from housekeeping genes showed that 14 Gallibacterium isolates fell into the same branch with G. anatis type strains. 14 Gallibacterium isolates were classified in G. anatis. In the three gene locus of gyrB, rpoB and 16S rRNA genes, there were no apparent genetic difference between Gallibacterium strains isolated from Henan and Shanxi Provinces, and from healthy chickens and diseased chickens with salpingitis. gyrB gene could be used for classification of Gallibacterium isolates, furthermore, more obvious differences was noticed in the homology analysis based on gyrB gene sequence between Gallibacterium isolates and G. genomosp. 1 than that based on 16S rRNA and rpoB genes.