Abstract:

The experiment was conducted to study the effects of 18carbon fatty acids (18CFAs) on cell proliferation and triacylglycerol accumulation in bovine mammary epithelial cells cultured in vitro. The cells were cultured with different concentration of stearic acid, oleic acid, linoleic acid and linolenic acid for 24 h at 38 ℃, and then the MTT experiment was performed to detect the cell proliferation and the triacylglycerol contents in medium were detected by Triglyceride Quantitation Kit. The results showed that the cell proliferation was inhibited significantly when the cells were cultured with 200 or 400 μmol·L-1 stearic acid, oleic acid, linoleic acid or linolenic acid (P<0.05 or P<0.01). The contents of triacylglycerol in medium were increased in a concentrationdependent manner from 0 to 100 μmol·L-1 (P<0.05 or P<0.01). In conclusion, high concentration of 18CFAs can inhibit the cell proliferation, and triacylglycerol accumulation can be increased as the concentration increase of 18CFAs.