Abstract:

This experiment was conducted to obtain and analyze cDNA sequence of goat MSX2, and to analyze the sequence character and expression rule. By reverse transcription PCR, the fulllength cDNA of MSX2 was cloned from skin of Shanbei White Cashmere goat, and the expression of MSX2 in different development periods of follicle were examined by quantitative realtime PCR. The results showed that the cDNA of goat MSX2 contained 804 nucleotides, including a complete open reading frame(ORF) encoding 267 amino acids. The amino acid sequence shared 96%, 90%, 89%, 88%, 87% and 85% identity with those of Bos taurus, Homo sapiens, Canis familiaris, Pan troglodytes, Rattus norvegicus, Mus musculus, respectively. The result of realtime quantitative PCR showed that MSX2 were higher expressed in April and November than that in other months (P＜0.05). The amino acid sequences of MSX2 were highly conservative. MSX2 was highly expressed at April and November, and it was expressed in different extent in the other months, which indicated that MSX2 gene might be involved in molecular regulation of hair growth cycle.