ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (5): 838-842.doi:

• 研究简报 • Previous Articles    

Cloning and Sequence Analysis of Goats Endogenous Pulmonary Adenomatosis Retrovirus

ZHOU Yanxi1 ，LI Jing1，AO Weihua2 ，LIU Xiaohui1，YU Lixin1, YAO Hongqiang1，MA Xueen1*   

  1. 1. College of Veterinary Medicine，Inner Mongolia Agricultural University, Huhhot 010018, China；2. Inner Mongolia EntyExit Inspection and Quarantine Bureau, Huhhot 010020, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28
  • Contact: MA Xueen

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Abstract: Three pairs of primers were designed according to the sequence of strain enJSRV20. Sequence of goats enJSRV was amplified by PCR technology and these PCR products were cloned into PMD18T vector and then sequenced . The complete genome of goats enJSRV was obtained successfully. Analysis results showed that the sequence of goats enJSRV was 93.4% similarity with enJSRV18 and 88.1% similarity with strain JS7, respectively. Phylogenetic analysis according to pro gene showed that goats enJSRV had higher homology with strain enJSRV5 .It will be useful for the study of constructing infectious clone and elucidation the function of endogenous pulmonary adenomatosis retrovirus.