ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (5): 740-747.doi:

• 动物营养 • Previous Articles     Next Articles

Effect of Zinc Lactate on Cell Proliferation and Relatedregulatory Genes mRNA Expression in Porcine Jejunal Epithelial Cells IPECJ2

HAN Guoquan1, YU Bing1，2, CHEN Daiwen1，2*, XIANG Zhentian1, QI Hongwei1, CHEN Hong1, MAO Qian1, MAO Xiangbing1, HUANG Zhiqing1   

  1. 1. Institue of Animal Nutrition, Sichuan Agricultural University, Ya’an 625014, China; 2. Key Laboratory of Animal Diseaseresistant Nutrition of Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-05-28 Published:2012-05-28

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Abstract: The present study was conducted to evaluate the effects of zinc lactate on cell proliferation of jejunal epithelial cells IPECJ2 and mRNA expression of relatedregulatory genes in porcine. IPECJ2 cells were cultured with mediums added with zinc lactate by the zinc concentrations of 50, 100, 150 and 200 mg·L-1, respectively. Cell proliferation was estimated by Colorimetric analysis. Simultaneously, realtime quantitative RTPCR was applied to detect the mRNA expression of relatedregulatory genes (ZnT2, DMT1, IREG1, MT1 and ZIP4), and TBP mRNA level was used as the control. During the first 36 hours after treatment with zinc lactate, there was almost no effect on IPECJ2 cell proliferation. Subsequently, with increasing of the concentration of zinc, the rate of cell proliferation gradually increased. At the same time, after IPECJ2 cell was treated with zinc lactate, the expression of ZnT2, DMT1, IREG1and MT1 mRNA was increased with the increase of the concentration of zinc while the expression of ZIP4 mRNA decreased. Zinc lactate could promote cell proliferation of IPECJ2, upregulated the mRNA expression of ZnT2, DMT1, IREG1, MT1 and downregulated the mRNA expression of ZIP4.