ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (2): 275-281.doi:

• 预防兽医 • Previous Articles     Next Articles

Isolation and Identification of Group A Porcine Rotavirus

KU Xugang1,2，ZHANG Kun1,2，LIU Yuxi1,2，HE Qigai1,2*   

  1. 1. State Key Laboratory of Agriculture Microbiology of Animal Virology，Wuhan 430070, China; 2. College of Animal Science and Veterinary Medicine, Huazhong Agricultural University，Wuhan 430070, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15
  • Contact: HE Qigai

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Abstract: Porcine rotavirus can induce serious diarrhea in piglet, but most researches are on human. We successfully isolated and identified group A porcine rotavirus (GAR) virus, which make a contribution to pathogenesis, molecular biology study on porcine rotavirus. GAR were detected by RTPCR test from different feces samples of diarrhea, those positive samples were treated by trypsin, then was inoculated to MA104 cell line to isolate strain TMa. After routine RTPCR test and electron microscope observation, we sequenced and analyzed VP6, VP7 and VP8 genes of strain TMa. Through homology analysis, we found VP6, VP7 and VP8 are, respectively, identical to Beijing human strain LL3354 from China (96.0%), Indian human strain RMC321 (95.1%), wild boar strain GUB71 from Japan (97.0%). Strain TMa have a high similarity to human and wild boar rotavirus, we infer that GAR may spread from human to animal, and there exist gene recombination.