ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (2): 270-274.doi:

• 预防兽医 • Previous Articles     Next Articles

Expression of Compound Multiepitope Gene of Classical Swine Fever Virus in E. coli and the Assessment of Its Immunologicity

TIAN Hong, HOU Xiangmin, WU Jinyan, CHEN Yan, SHANG Youjun, LIU Xiangtao*   

  1. Key Laboratory of Animal Virology of Ministry of Agriculture, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15
  • Contact: LIU Xiangtao

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Abstract: Genetical engineer T, B cell epitopes of Classical swine fever virus (CSFV) were developed by employing molecular biological tools, and their immunogenicity has been primarily evaluated by immunizing experimental animals. A DNA fragment which encoding a tandem repeat protein of T, B cell epitopes of CSFV were designed and chemically synthesized, and it was cloned into pGEX6P1 vector in turn to form a recombinant plasmid pGEXBT500. A chimeric protein was obtained after transforming the pGEXBT500 into Escherichia coli BL21 (DE3) host cell and induced by IPTG. The western blotting analysis showed that the expressed products have a good reactogenicity, which can react specially with CSF positive sera. Inoculation with 100 μg recombinant protein induced strong neutralizing antibody response in rabbits. Our study indicated that the expressed T, B epitopes can act as the carrier protein for CSFV peptide epitopes, and this recombinant protein is a potential multiepitope peptide vaccine candidate to prevent CSFV infection.