ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (2): 250-254.doi:

• 动物营养 • Previous Articles     Next Articles

Effects of Lactobacillus rhamnosus on Antioxidative Functions and Cytokines Secretion in Caco2 Cells

HUANG Yi1,2, HUANG Qin1, LI Weifen1*, YU Dongyou1, ZHOU Xuxia1   

  1. 1. Key Laboratory of Animal Molecular Nutrition of Ministry of Education, Colleg of Animal Science, Zhejiang University, Hangzhou 310058, China; 2.College of Animal Science and Technology， Guangxi University, Nanning 530005, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15

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Abstract: In this study, the effects of Lactobacillus rhamnosus on antioxidative and nonspecific immunologic functions in Caco2 cells were investigated. Caco2 cells were treated by PBS(group A), E. coli K88(group B) and L. rhamnosus (group C), respectively. Caco2 cells in group D were preincubated with L. rhamnosus and then infected with E. coli K88. The results showed that TAOC concentration decreased（P＜0.01）, but SOD activity enhanced in cell culture supernatant（P＜0.01）in group B. However, the adverse situation appeared in group C and, SOD activity in cell lysate increased（P＜0.05）. APRIL secretion were induced（P＜0.01）, however, IL8 secretion were inhibited（P＜0.01）both in group B and group C in different degree. IL10 production was stopped in group B, and was promoted in group C（P＜0.01）. Compared with group B, APRIL production significantly decreased（P＜0.01）, whereas levels of IL8 （P＜0.05）and IL10（P＜0.01）increased in group D. These results suggested that Caco2 cells were sensitize to respond to L. rhamnosus by an improved response in antioxidative function. In addition, inflammatory response against pathogenic bacteria in enterocyte attenuated, moreover, immune response reinforced, which indicated the activities for immunoprotection and immunoadjuvant in L. rhamnosus.