Abstract: The aim of this study was to study the possible regulation mechanism of PIT1 promoter by searching for the cisregulatory elements, transacting factors and basic transcription units of the transcription regulation. The goose promoter of PIT1 gene was cloned by genome walking and subcloned into the luciferase expression vector pGL3Basic directly. A series of promoter missing mutants were constructed (-1 485/-1 bp，-1 293/-1 bp，-1 014/-1 bp，-775/-1 bp，-561/-1 bp，-353/-1 bp，-206/-1 bp), and identified by restrictive endonuclease enzyme cutting, PCR and sequencing. The recombinant plasmids were detected by SteadyGlo LuciferaseAssay system after transient transfecting GH3 cell. This study indicated that the PIT1 gene promoter cloned had obviously promoter activity. All of them, -561/-1 bp had highest activity, and many possible transcription factor binding sites such as (PIT1，POU3F2，myogenin and GR) were predicted. The pGLPIT1 promoter was constructed successfully by series missing method. The promoter cloned in the study had activity, the positive and negative regulation areas and core promoter region were found. Furthermore, the result provided the foundation for analyzing the promoter activity and transcriptional regulation mechanism.