ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (2): 211-219.doi:

• 遗传繁育 • Previous Articles     Next Articles

Construction of a Suppression Subtractive Hybridization cDNA Library to Screen Differentially Expressed Genes from Duck Liver Infected Duck Hepatitis Virus

LI Xiu1, XU Qi1, ZHANG Yang1, BI Yulin1, ZHAO Rongxue1,
CHEN Changyi2, DUAN Xiujun3, CHEN Guohong1*   

  1. 1 . College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China； 3.Jiangsu Animal Husbandry & Veterinary College，Taizhou 225300，China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15
  • Contact: CHEN Guohong

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Abstract: This research aimed to detect and identify genes associated with duck viral hepatitis(DVH) by construction of suppression subtractive cDNA library, and explore their mechanism by function cluster analysis. Using suppression subtraction hybridization(SSH), a different expression SSHcDNA library of 3dayold and fullsib ducklings infected artificially by DHV was constructed and the same dayage ducklings were injected with the same quantity of saline. Of which 563 clones were sequenced, and 299 differentially expressed sequence tags (ESTs) were obtained. After dislodging the redundant cDNA sequence and clustering splicing, the software BLAST in NCBI of GenBank was used to do the nucleic acid and protein homology comparisons and functional analysis. The results showed that 70 different genes had highly homologous with ESTs(E value <e-10,match length>150 bp,match rate>80%), most of which were related to the synthesis of cell components, signal transduction and the biological control process of pathological conditions. The occurrence and development of DVH was a complex multistep process involving multiple genes. The results provide a basis for further study of the molecular mechanism of DVH.