ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (2): 204-210.doi:

• 遗传繁育 • Previous Articles     Next Articles

Structural and Functional Analysis of Fatty Acid Synthase Gene Promoter of Goat

LI Jun1, GE Ting2, LUO Jun1*, SUN Yuting1, SHI Hengbo1, ZHU Jiangjiang1, HAO Juan1, ZHAO Wangsheng1   

  1. 1. Shanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University，Yangling 712100, China; 2. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2012-02-15 Published:2012-02-15
  • Contact: LUO Jun

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Abstract: The objective of this study was to analyze the structure and function of fatty acid synthase gene（FASN）promoter of goat, to further reveal the transcriptional regulatory mechanism of FASN gene. In this study, the promoter of goat FASN gene was obtained by PCR. Seven promoter fragments in different length were obtained by deletion and cloned into luciferase reporter gene expression vectors. Then, the vectors were transfected into goat mammary epithelial cells and MCF7 cells, their expression activity were determined by using the dualluciferase reporter assay system. The promoter sequence of 2 589 bp of FASN gene was obtained. The bioinformatics analysis for sequence showed that there were a TATAbox at -41 bp and an Ebox at -74 bp of transcription initiation site (+1). Luciferase reporter assays demonstrated that the core region of the promoter was from -293 bp to -79 bp conferring basal transcriptional activity. Meanwhile, some transcriptional factor binding sites including Sp1, NFY, USF and SREBP were identified in this core region. The results indicated that the transcriptional factors such as Sp1, NFY, USF and SREBP might be involved in the transcriptional regulation of FASN gene.