Abstract: To expound the development and specification of germ cells in fetal embryonic gonad is important for the goat breeding and reproduction, and can enrich the content of embryology. 22 goat embryonic gonads from 39 to 150 dpc (days post conception) were studied with histological methods , immunohistochemistry and immunofluorescence．The results showed that spermatic cord and tunica albuginea have already appear and sex differentiation have already occurred at 3947 dpc. The germ cells, Sertoli cells and leydig cells could be identified at that time. Before 50 dpc，the germ cell with small size and high nuclear/cytoplasmic ratio can be called gonocytes.The germ cells experienced dramatic change during 5159 dpc. Then gonocytes differentiating into prespermatogonia, which were predominant and nearly all of them expressed VASA. Germ cells at 5090 dpc had the maximum of proliferation rate,and percentage of germ cells reached its peak. Afterward the proliferation rate of germ cells was declined and leydig cells atrophy. Through this study,after 50 dpc the gonocytes differentiate into prespermatogonia, germ cells at 5090 dpc have the maximum of proliferation rate,VASA can be used as a marker of prespermatogonia.