ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (9): 1249-1255.doi:

• 遗传繁育 • Previous Articles     Next Articles

Prediction of Bovine Oocyte Developmental Capacity by Brilliant Cresyl Blue (BCB) Staining of COCs

QIAO Li-min 1, LI Xiang-chen4， QIAO Fu-qiang2, YAO Hua3, ZHANG Wen-xiu4, GUAN Wei-jun4*, MA Yue-hui4*

  

  1. 1. Department of Animal and Veterinary, Beijing Vocational College of Agriculture, Beijing 102442, China; 2. Group of Science Technology and Industry, Beijing University of Agriculture, Beijing 102206, China; 3. Animal Science and Technology College, Beijing University of Agriculture, Beijing 102206, China; 4. Institute of Animal Science, Chinese Academy of gricultural Sciences, Beijing 100193,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-23 Published:2011-09-23
  • Contact: GUAN Wei-jun, MA Yuehui

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Abstract: Bovine immature oocytes were stained by brilliant cresyl blue (BCB). Oocyte growth and apotosis between different groups was observed. The objective of the study was to investigate the feasibility of BCB staining applied to estimate bovine oocyte quality. Immature bovine oocytes were stained by 26 μmol·L-1 BCB for 90 min as experimental groups(BCB+ and BCB-) and unstained as control. Concentration of GSH in oocytes was as an index to determine cytoplasmic mature, and blastula rate was as an index to estimate oocytes growing potential after in vitro fertilization. The results showed: (1) The bovine COCs BCB+ oocyte was showed a higher mature rate than that in the BCB- and the control groups (P<0.05); (2) There was a significant difference in concentration of glutathione between mature and immature oocytes(P＜0.05); (3) There was significant difference in mature rate and apoptosis rate between BCB+ group and the control and BCB- groups(P<0.05); (4) The BCB+ showed a higher blastula rate and the more blastular cells than that of BCB- and the control groups after in vitro fertilization(P<0.05). The result indicate that bovine oocyte stained by BCB could be a marker to evaluate the developmental ability of bovine oocyte in the future.