ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (9): 1233-1238.doi:

• 遗传繁育 • Previous Articles     Next Articles

The Influence on mRNA Expression of Maternal Genes in Ovine Oocytes by Vitrified Freezing

WANG Jing1,2,LIN Jia-peng2, SHI Qing-hua1,CHEN Bo1,2,WANG Li-qin2,ZHAO Yun-cheng2, HUANG Jun-cheng2*

  

  1. 1. Xinjiang Academy of Biotechnological Center, the Key Lab of Livestock Reproduction & Breeding Biotechnology of MOA, Xinjiang Academy of Animal Science, Urumqi 830000, China; 2. College of Agriculture,Xinjiang Agricultural University, Urumqi 830052, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-09-23 Published:2011-09-23
  • Contact: HUANG Jun-cheng

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Abstract: To investigate the effect on mRNA expression of maternal genes in ovine oocytes by vitrified freezing, the ovine oocytes at stage GV and IVM(18,24 h) were disposed with the method of vitrified freezing and cultured in vitro under normal conditions after defrosting, and the result showed that the vitrified oocytes at stage GV and IVM both had lower cleavage rate (10.37%, 23.17%, 33.07%) than that in control group (82.96%，P<0.01）.In addition, vitrified oocytes at stage GV had lower cleavage rate than that at stage IVM (P<0.01) .The content of mRNA of four maternal genes, Gdf9 (growth differentiation factor9), Zar1 (zygote arrest factor1), Mater (maternal antigen that embryo requires ), Dnmt1 (DNA methyltransferase1) in ovine oocytes treated differently were detected by fluorescent quantitative realtime PCR in this experiment. The results showed that the content of the four genes mRNA increased using vitrification,especially that in oocytes at stage GV was the highest (P<0.01). The result indicate that the bovine oocytes at stage GV or IVM with higher transcripts by vitrification, may have negative effect on embryonic development.