ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (4): 578-584.doi:

• 研究简报 • Previous Articles     Next Articles

Screening and Analyzing of Genes on Prolificacy in Goat

QIAN Li-li1, JIA Qing1,2*, LI Xue-mei1，LI Xun-ye3, ZHENG Hui-qin1, FENG An-xue1   

  1. 1.College of Animal Science and Technology, Agricultural University of Hebei,Baoding 071001,China; 2. National Engineering Research Center for Agriculture in Northern Mountainous Areas, Baoding 071001,China; 3. Shenzhou Bureau of Animal Industry, Shenzhou 052800, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-04-20 Published:2011-04-20

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Abstract: To search the genes associated with prolificacy of goats, and research the genetic mechanism of fertility regulation, twelve Jizhong goats were divided into 2 groups according to their reproductive records. Differential display reverse transcript PCR (DDRT-PCR) method was used in the analysis of the discrepancy of gene expression in ovarian tissue of goats in estrual period 4 segments differentially expressed in quantity were finally obtained via second PCR amplification, reverse Northern blot and semi-quantitative PCR After sequencing and cloning, these segments were submitted to GenBank, and analysised by BLAST. Two of the 4 were new segments and high homologous sequences in NCBI was not found. Another segment shared 81% similarity with one clone segment (THY010003E05) of wild boar (Sus scrofa), but with unknown function. The other segment was 94% similar to the mRNA of PCNP gene in cattle. It indicate that the segment is a homolog of PCNP gene and it could affect goat prolificacy via involving in cell cycle regulation of oocyte according to the analysis of its function.