ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (2): 203-209.doi:

• 预防兽医 • Previous Articles     Next Articles

Continuously Expressing HIV-1 Gag and EGFP by Retroviral Vector InducedSP2/0 Cell Lines

WANG Jing1, LI Chang2, LI Lin-xi1, HU Bo1, CONG Yan-zhao3,
REN Da-yong1, WANG Zhuo-yue1, DU Shou-wen1, JIN Ning-yi1,2*   

  1. 1.College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China; 2.Genetic Engineering Laboratory of PLA, Academy of Military Medical Sciences of PLA, Changchun 130062, China; 3. College of Agriculture,Yanbian University, Longjing 133400, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-25 Published:2011-02-25

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Abstract: To construct a retroviral vector mediated mammalian cell expression cell line of the human immunodeficiency virus (HIV-1) core protein, the recombinant retroviral vector pFB-gag-EGFP was constructed by inserting core protein gene gag of HIV-1 and enhanced green fluorescent protein gene EGFP into pFB-neo by turns. The recombinant retroviral vector, the pVPack-GP plasmid containing the helper virus gag-pol gene and pVPack-10A1 plasmid containing env gene were cotransfected into packaging cell line HEK-293T with liposome-mediated transduction, and then the recombinant retrovirus was collected and infected SP2/0 cells. The green fluorescent protein was observed under fluorescence microscope, and the expression of core protein Gag of HIV-1 was verified. The resistance cells were screened by G418. The core protein Gag of HIV-1 and green fluorescent protein could express in SP2/0 cells, and the mammalian cell line of the core protein gene gag of HIV1 had been constructed successfully, which laid a mouse cell model of activity assay of anti.AIDS genetic engineering therapeutic agents and targeted drugs.