Abstract: To further understand the biological function of goat PTHrP gene, the PTHrP polyclonal antibody was prepared and the tissue expression profile of PTHrP was analyzed in this study. Prokaryotic expression vector was constructed and expressed in E-coli BL21(DE3) host cells. Fusion protein was purified and used to immunize rabbit. Western blot was performed to assay the tissue expression profile. Restriction enzyme mapping and sequencing showed that prokaryotic expression vector was constructed successfully. SDS-PAGE showed that fusion protein (about 36.5 ku) was purified. The anti-PTHrP polyclonal antibody showed high sensitivity(1︰51 200) and specificity. The PTHrP protein was detected in mammary gland, kidney, pituitary, brain, heart, liver, bone, muscle and spleen.The result indicated that goat anti-PTHrP polyclonal antibody was obtained. The PTHrP expressed widely in goat tissues.