ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (2): 163-168.doi:

• 遗传繁育 • Previous Articles     Next Articles

Pedigree Verification of Holstein Cows and Analysis of Factors Affecting Incorrect Paternity Based on Microsatellite Markers

CHU Qin1,2, ZHANG Yi1, SUN Dong-xiao1, YU Ying1, WANG Ya-chun1, ZHANG Yuan1*   

  1. 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China;2. Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-25 Published:2011-02-25

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Abstract: To verify the pedigree data of Chinese Holstein cows,17 microsatellites on BTA3 were genotyped in 15 Chinese Holstein sires and their 2 220 daughters, which came from 21 herds in Beijing. The paternity with conflicts between the putative sire and daughter in at least 3 markers was considered to be erroneous. The paternity error rate was 16.62% in the Chinese Holstein population. Factors affecting incorrect paternity were further analyzed and results showed that the paternity error rate varied extremely significantly among herds. Since the daughters of sires were uneven distributed in different farms, the effect of sire was also significant. These findings indicate that it’s urgent to carry out paternity testing in the Chinese Holstein population.