ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (11): 1615-1619.doi:

• 临床兽医 • Previous Articles     Next Articles

Analysis on the Dynamic Changes of Lymphocyte Subsets and Genetic Transcription of Cytokine IL-2, IL-4 in Peripheral Blood of Dairy Cows during Perpartum

CUI Qunwei1, GUO Ning1, ZHOU Xuan1, WANG Zhenyun1, WANG Yachun2*, WANG Genlin1*   

  1. 1．College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China; 2．College of Animal Science and Technology, China Agricultural University,Beijing 100193, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
  • Contact: WANG Yachun2*, WANG Genlin1*

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Abstract: To reveal the mechanism of impaired immune function in perpartum period, the dynamic changes of CD3+, CD4+ and CD8+ in lymphocyte subsets as well as gene transcription level of cytokine IL2 and IL4 in peripheral blood of dairy cows during 21 days before calving to 21 days after it were investigated in the present study. The dynamic changes of Lymphocyte cells CD3+, CD4+, CD8+ and the mRNA expression of cytokine IL2, IL4 in peripheral blood were determined by flow cytometry and RTPCR respectively. The results showed that significant difference was found in the proportion of CD3+ in peripheral blood 21 days before calving (50.8±4.61)% and 14 days after caving (39.21±4.98)%, and also was found in the proportion of CD4+ in blood before (21.38±0.65)% and after (6.89±0.32)% calving. The proportion of CD8+ increased to the highest level (16.9±2.02)% during calving (P<0.05). The ratio of CD4+/CD8+ was >1 before calving, and <1 after calving. The mRNA expression of IL2 was the lowest (P<0.05) during 014 days after calving, then gradually increased. The mRNA expression of IL4 increased to the peak during calving, and declined to the lowest level on the 7th day (P<0.01). It was suggested that the immune function of dairy cows was impaired within 21 days before and after calving, especially during the 014 days after caving. This might be caused by the quantity and ratio changes of lymphocyte subsets (CD3+, CD4+and CD8+) and the downregulation of the cytokine IL2, IL4 during this critical period.