ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (11): 1577-1583.doi:

• 预防兽医 • Previous Articles     Next Articles

Influence of the Mutations of VP2 on the Replication Efficiency of Infectious Bursal Disease Virus in vitro

QI Xiaole1,2, GAO Li2, WU Guan2, DENG Xiaoyun2, 3, YU Fei2, ZHANG Lizhou2, QIN Liting2, GAO Yulong2, WANG Yongqiang2, GAO Honglei2, LIU Di1, HUA Yuping1, WANG Xiaomei2*   

  1. 1. Postdoctoral Workstation of Heilongjiang Academy of Agricultural Science, Postdoctoral Station of Northeast Forestry University, Harbin 150086, China; 2. Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China; 3. Heilongjiang Vocational College of Science and Technology, Shuangcheng 150111, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
  • Contact: WANG Xiaomei

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Abstract: To study the molecular basis for the replication efficiency and virulence of infectious bursal disease virus (IBDV), two amino acid mutations (A222P, D279N) was introduced in to VP2 of IBDV, respectively, based on the information of sequence analysis. Using the reverse genetic research system, two responding mutated strains of IBDV were rescued and their biological characteristics were studied. The replication kinetic curves showed that the rescued virus including A222P replicated over 6 times higher than its parent strain did, while D279N couldn’t. Animal experiment of SPF chickens showed that residue 222 and 279 of VP2 did not contribute to virulence of IBDV. The relations between the two residues of VP2 (222, 279) and the replication and virulence of IBDV were reported firstly. The findings provide necessary information for further understanding the gene function of IBDV and the design of new tailored IBDV vaccines.