ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2011, Vol. 42 ›› Issue (11): 1526-1531.doi:

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Light Microscope Immunocytochemical Studies of the Distribution of Caspase-9 in the Reproductive Organs of Meat Sheep

ZHANG Taojie1, CUI Yan1, YU Sijiu1,2*, FAN Jiangfeng2, LIU Ben3, ZHENG Kai1   

  1. 1. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China; 2. Technology and Research Center of Gansu Province for Embryonic Engineering of Bovine and Sheep & Goat, Lanzhou 730070,China; 3. Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-11-25 Published:2011-11-25
  • Contact: YU Sijiu

Abstract:

The expression of the apoptotic gene caspase9 in the reproductive organs of meat sheep was observed and its physiological significance was determined by using immunohistochemistric technology. Caspase9 was only found in the cytoplasm. The majority of positive cells in the ovary were observed in primordial and primary follicles.In the later luteal phase, Caspase9 was positive in granular lutein cells but negative in theca lutein cells;during the luteal phase, Caspase9 was positive in endometrial epithelium and shallow glands of the oviduct, the lymphatic nodule of the cercix uteri, the ciliated cell and secretory cell of the mucous epithelium in ampulla tubae uterinae ,and the epithelium of serous gland of the tubal isthmus; during the follicular phase, no obvious positive expression was found. All results mentioned above indicate that Caspase9 participate in the regulation of apoptosis and immunological tissue of the major reproductive organs of meat sheep. It is important for the stability of reproductive organs in meat sheep.