Abstract: In order to investigate the excretion quantitation of main metabolites of mequindox in chicken feces，a high performance liquid chromatography method was developed to detect the concentrations of metabolites M1, M2 of mequindox. Fifteen seven-week-old chickens were administered mequindox orally at a dose of 30 mg·kg^-1 BW. After administration, all of their feces were collected at different time intervals and weighed. Then 2 g of feces were extracted using methylene chloride/acetonitrile (1︰1 V/V) solution and the organic phase was separated and evaporated. The residual was redissolved with methanol/water (30︰70 V/V) solution, and then analyzed by HPLC. The results showed that the limit of detection (LOD) and limit of quantitation (LOQ) of metabolites M1 and M2 were 0.01 and 0.05 μg·mL^-1, respectively. The range of average recovery of metabolites M1 and M2 were 69.5%-72.4%, 73.5%-80.6% under the concentrations of 0.05, 1.0 and 50 μg·mL^-1. The proportions of metabolites M1 and M2 in total amount of mequindox administered were 3.20% and 4.58%, respectively. The excretion peak time of metabolite M1 was 4.8 h with 38.09% of accumulated excretion total amount of metabolite M1. And the excretion peak time of metabolite M2 was 12.24 h with 42.29% of accumulated excretion total amount of metabolite M2 after administration. The present results indicated that the method developed in this study could be used to detect the concentration of metabolites M1 and M2 of mequindox in chicken feces.