ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2010, Vol. 41 ›› Issue (9): 1208-1212.doi:

• 研究简报 • Previous Articles     Next Articles

Analysis of Genetic Diversity in Ritu Tibetan Goats by ISSR

WANG Yong1*, WANG Jie1, XU Qi-shu1, ZI Xiang-dong1, OUYANG Xi1, LIU Lu-shu1, XIAO Yi-xi2   

  1. 1. Sichuan Provincial KeyLaboratory of Protection and Utilization of Animal Genetic Resources in QinghaiTibet Plateau, Southwest University for Nationalities, Chengdu 610041, China; 2. Academy of Agriculture and Animal Husbandry of Tibet Autonomous Region, Lasa 850000， China
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-09-20 Published:2010-09-20

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Abstract: Inter simple sequence repeat(ISSR)was used to analyze the genetic diversity of Tibetan goats population in Ritu county. Ten primers were selected from 93 ISSR primers and then used to detect the genetic diversity in 107 Tibetan goats. The obtained data were analyzed by using Excel 2003 and SPSS11.0 softwares. Ten out of 93 ISSR primers produced 112 DNA bands, among them 75 bands were polymorphic markers and the size of amplified fragment ranged from 219 to 2 534 bp. It indicated that the selected primers were specific and could produce enough polymorphic bands. The proportions of polymorphic loci(P)in Ritu Tibetan goats was 66.96%. The genetic similarity index(s)within the detected population ranged from 0.460 0 to 0.740 5(with average of 0.564 7). The averaged individual genetic difference(D)was 0.435 3. The gene frequency(f)ranged from 0.045 5 to 1.000 0. These results of ISSR analysis indicated that the Ritu Tibetan goats population presents high level of genetic diversity and genetic variations existed among individuals.