Abstract: From October 2008 to September 2009, 2 420 cloaca swab samples were collected from domestic poultry in the LPMs (livepoultry markets), and 83 strains of AIVs were isolated with an isolation rate of 7.36％. These strains of AIV belonged to 7 HA subtypes, from high to low, the order of the isolation rate was H6, H3, H4, H9, H11, H1, H8. The isolation rate of AIVs was distributed with significant seasonal variations, higher in winter and spring and lower in summer. The distribution of HA subtypes of AIVs was also uneven in different month. Waterfowl are considered as the important host of influenza A viruses, especially domestic ducks. So far, 8 HA subtypes (H1, H3, H4, H6, H8, H9, H10 and H11) and 7 NA subtypes (N1, N2, N3, N4, N5, N6 and N8) were isolated from domestic ducks. Strains of HA and NA subtypes assembled 18 subtypes. Domestic duck is still the most prone to mixed infections, and H6 subtypes played the main role, H3 and H9 were the other main mixed infection subtypes, providing a good carrier to generate a new subtype and virulence variation.